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51.
Jun TAMURA Takaharu ITAMI Tomohito ISHIZUKA Sho FUKUI Kenjirou MIYOSHI Tadashi SANO Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):865-869
To determine the reference level of central venous oxygen saturation (ScvO2)
and clinical efficacy of central venous blood gas analysis, partial pressures of oxygen
and carbon dioxide, pH, oxygen saturation, base excess (B.E.) and HCO3
concentration were compared between simultaneously obtained central venous and arterial
blood samples from conscious healthy 6 dogs and 5 cats. Comparisons between arteriovenous
samples were performed by a paired t-test and Bland-Altman analysis.
Between arteriovenous samples, B.E. showed good agreement, but there were significant
differences in other parameters in the dogs, and no good agreement was detected in cats.
The ScvO2 in dogs and cats were 82.3 ± 3.5 and 62.4 ± 13.5%, respectively.
Central venous blood gas analysis is indispensable, especially in cats. 相似文献
52.
Kazuya KUSHIDA Urs GIGER Toshihiko TSUTSUI Megumi INABA Yoshio KONNO Kureha HAYASHI Kana NOGUCHI Akira YABUKI Keijiro MIZUKAMI Moeko KOHYAMA Yasuyuki ENDO Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):743-746
Erythrocyte pyruvate kinase (PK) deficiency is an inherited glycolytic erythroenzymopathy
caused by mutations of the PKLR gene. A causative mutation of the feline
PKLR gene was originally identified in Abyssinian and Somali cats in
the U.S.A. In the present study, a TaqMan probe-based real-time PCR genotyping assay was
developed and evaluated for rapid genotyping and large-scale screening for this mutation.
Furthermore, a genotyping survey was carried out in a population of four popular purebred
cats in Japan to determine the current mutant allele frequency. The assay clearly
displayed all genotypes of feline PK deficiency, indicating its suitability for
large-scale survey as well as diagnosis. The survey demonstrated that the mutant allele
frequency in Abyssinian and Somali cats was high enough to warrant measures to control and
prevent the disease. The mutant allele frequency was relatively low in Bengal and American
Shorthair cats; however, the testing should still be carried out to prevent the spread of
the disease. In addition, PK deficiency should always be considered in the differential
diagnosis of anemia in purebred cats in Japan as well as worldwide. 相似文献
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Takafumi SUZUKI Naohito NISHII Satoshi TAKASHIMA Tatsuya MATSUBARA Atsushi IWASAWA Hirofumi TAKEUCHI Kohei TAHARA Tatsuyuki HACHISU Hitoshi KITAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1379-1383
Polyclonal immunoglobulin (Ig) G autoantibodies against insulin have been
identified in sera of healthy cats. We purified and fractionated insulin-binding IgGs from
cat sera by affinity chromatography and analyzed affinity of insulin-binding IgGs for
insulin and their epitopes. Following the passing of fraction A, which did not bind to
insulin, insulin-binding IgGs were eluted into two fractions, B and C, by affinity
chromatography using a column fixed with bovine insulin. Dissociation constant (KD) values
between insulin-binding IgGs and insulin, determined by surface plasmon resonance analysis
(Biacore™system), were 1.64e−4 M for fraction B (low affinity IgGs) and
2e−5 M for fraction C (high affinity IgGs). Epitope analysis was conducted
using 16 peptide fragments synthesized in concord with the amino acid sequence of feline
insulin by an enzyme-linked immunosorbent assay. Fractions B and C showed higher
absorbance (affinity) of the peptide fragment of 10 amino acid residues at the
carboxyl-terminal of the B chain (peptide No. 19), followed by peptide fragments of 6 to
15 amino acid residues of the B chain (peptide No. 8). Fraction C showed a higher
absorbance to 7 to 16 amino acid residues of the B chain (peptide No. 5) compared with the
absorbance of fraction B. Polyclonal insulin-binding IgGs may form a macromolecule complex
with insulin through the multiple affinity sites of IgG molecules. Feline insulin-binding
IgGs are multifocal and may be composed of multiple IgG components and insulin. 相似文献
55.
Comparison of the effects of propofol or alfaxalone for anaesthesia induction and maintenance on respiration in cats 下载免费PDF全文
Ivo Campagna Andrea Schwarz Stefanie Keller Regula Bettschart‐Wolfensberger Martina Mosing 《Veterinary anaesthesia and analgesia》2015,42(5):484-492
ObjectiveTo compare the effects of propofol and alfaxalone on respiration in cats.Study designRandomized, ‘blinded’, prospective clinical trial.AnimalsTwenty cats undergoing ovariohysterectomy.MethodsAfter premedication with medetomidine 0.01 mg kg−1 intramuscularly and meloxicam 0.3 mg kg−1 subcutaneously, the cats were assigned randomly into two groups: group A (n = 10) were administered alfaxalone 5 mg kg−1 minute−1 followed by 10 mg kg−1 hour−1 intravenously (IV) and group P (n = 10) were administered propofol 6 mg kg−1 minute−1 followed by 12 mg kg−1hour−1 IV for induction and maintenance of anaesthesia, respectively. After endotracheal intubation, the tube was connected to a non-rebreathing system delivering 100% oxygen. The anaesthetic maintenance drug rate was adjusted (± 0.5 mg kg−1 hour−1) every 5 minutes according to a scoring sheet based on physiologic variables and clinical signs. If apnoea > 30 seconds, end-tidal carbon dioxide (Pe′CO2) > 7.3 kPa (55 mmHg) or arterial haemoglobin oxygen saturation (SpO2) < 90% occurred, manual ventilation was provided. Methadone was administered postoperatively. Data were analyzed using independent-samples t-tests, Fisher's exact test, linear mixed-effects models and binomial test.ResultsManual ventilation was required in two and eight of the cats in group A and P, respectively (p = 0.02). Two cats in both groups showed apnoea. Pe′CO2 > 7.3 kPa was recorded in zero versus four and SpO2 < 90% in zero versus six cats in groups A and P respectively. Induction and maintenance dose rates (mean ± SD) were 11.6 ± 0.3 mg kg−1 and 10.7 ± 0.8 mg kg−1 hour−1 for alfaxalone and 11.7 ± 2.7 mg kg−1 and 12.4 ± 0.5 mg kg−1 hour−1 for propofol.Conclusion and clinical relevanceAlfaxalone had less adverse influence on respiration than propofol in cats premedicated with medetomidine. Alfaxalone might be better than propofol for induction and maintenance of anaesthesia when artificial ventilation cannot be provided. 相似文献
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58.
H. Petznek M. Kleiter A. Tichy A. Fuchs-Baumgartinger C. Hohenadl 《Research in veterinary science》2014
Therapy of cats suffering from feline injection site sarcomas (FISS) is still a challenging problem, as the recurrence rate after surgery is up to 70%. Four FISS-derived primary tumour cell lines and corresponding xenograft tumour mouse models were established to evaluate the efficacy of a concomitant chemo-/radiation therapy with doxorubicin. In vitro, strongly depending upon the timing of administration, pre-treatment with 0.25 µmol doxorubicin resulted in a significant enhancement of radiation-induced (3.5 Gy) tumour cell death. This result was confirmed in vivo, where only the combined chemo-/radiation therapy resulted in a significant reduction in tumour growth compared to the respective mono-therapies with either doxorubicin or radiation. These results support the use of the concomitant chemo-/radiation therapy for adjuvant treatment of FISS, particularly in advanced or recurrent disease where surgery alone is no longer feasible. 相似文献
59.
?zge Turna YILMAZ T. Seval Fatma TOYDEMIR ?smail KIR?AN Banu DOKUZEYLUL Zeynep GUNAY Esra KARACAM 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(12):1595-1601
The analgesic effect of wound
infiltration with bupivacaine was evaluated in cats undergoing bilateral mastectomy.
Twenty-one female cats with mammary gland tumors were anesthetized with propofol and
oxygen-isoflurane anesthesia following premedication with atropine. In the trial group
(Group I; n=11), 30 ml of saline containing 2 mg/kg of bupivacaine was
infiltrated topically into the surgical wound right after removal of the mammary glands,
whereas only saline solution was infiltrated in the control group (Group II; n=10). At the
same time, carprofen (4 mg/kg) was also administered subcutaneously in both groups.
Behavioral signs of pain were monitored during the recovery period after general
anesthesia. In order to examine the behavioral changes associated with acute pain, a
questionnaire was prepared and given to the owners to be completed 4 hr and then 10 hr
after the operation. According to the owners’ anwers to the questionnaire, a pain score
was specified using a “numerical rating scale” for each cat. Although some cats showed
mild to moderate pain, the pain score recorded at 4 hr after the operation was
significantly lower in Group I (P<0.001). No significant difference
was found at 10 hr after the operation between the groups. The incidence of vocalization,
aggression and convulsion within 2 hr after the operation was also lower in Group I. In
conclusion, wound infiltration with bupivacaine before incisional closure provided
reliable analgesia at least 4 hr after bilateral radical mastectomy in cats. 相似文献
60.